Spring 2025 Co-op: Guide RNA Chemistry

Co-op Assignment Overview

Prime Medicine is seeking a Co-Op for the Spring 2025 semester with strong knowledge in chemistry/biochemistry to join our guide RNA chemistry team. The qualified candidate will be involved in the synthesis, purification, and analysis of oligonucleotides. In this position, you will gain comprehensive knowledge and hands-on experience in solid-phase synthesis, purifications, desalting, lyophilization, and analysis of long oligonucleotides for supporting prime editing programs.

Primary Responsibilities:

• Support solid-phase synthesis of oligonucleotides using automated DNA/RNA synthesizers.
• Assist in HPLC purification and UPLC and LCMS analysis of research scale long oligonucleotides.
• Preparing buffers and solutions for guide RNA purification and analysis.
• Track and manage chemical, reagent, and consumables inventory and ordering.
• Assist with maintenance and troubleshooting lab equipment such as synthesizers, speedvacs, lyophilizers, and HPLC instrumentation.
• Maintain a well-documented electronic laboratory notebook (ELN). Track progress, analyze, interpret, and present data within the team and cross-functional teams.
• Conducting lab work safely and efficiently, ensuring a safe working environment for colleagues.

Qualifications:

• B.S. candidate in Chemistry/Biochemistry or related subjects.
• Prior experience working in a chemistry or biochemistry laboratory.
• A high degree of flexibility and self-motivation in a fast-paced work environment with excellent organizational skills and the ability to prioritize appropriately and meet timelines.
• Excellent troubleshooting and communication skills.
• Ability to work independently and within a team.

Preferred Qualifications

• Chemistry or Biochemistry major with basic knowledge of organic chemistry.
• Basic knowledge of common computer programs (Excel, Work, and PowerPoint).
• Independently motivated, detail-oriented, and excellent problem-solving abilities.
• Prior experience in nucleic acid chemistry is a plus.
• Hands-on experience with HPLC, UPLC, and LCMS is a plus.

What We Offer:

• A cutting-edge team open to mentorship and networking.
• Full reimbursement for monthly commuting costs including MBTA, Commuter Rail, or Bluebikes, etc.
• Participation in regular seminars with industry experts.
• Full participation in employee activities including learning and development offerings, social activities, etc.

NOTE: We are currently not considering co-op candidates outside of the local Boston/Cambridge area.