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Senior Scientist I
Neuromuscular Disorders, Gene Editing
Posted on 12/30/2022
INACTIVE
Locations
San Bruno, CA, USA
Experience Level
Entry
Junior
Mid
Senior
Expert
Requirements
  • PhD in a life sciences discipline
  • 4+ years of relevant research post-PhD experience in an industry or academic setting
  • Experience with small molecule, gene therapy or nucleic acid therapy modalities for neuromuscular diseases
  • Deep knowledge of genetic neuromuscular diseases and relevant in vitro and animal models of disease, including target disease validation
Responsibilities
  • Contribute to the research efforts on designing, testing, optimizing and selecting lead candidates for neuromuscular indications using our novel Mammoth Cas nuclease systems
  • Lead from the bench junior and senior researchers to generate data to address neuromuscular disease, from proof-of-concept to development candidates
  • Collaboratively lead and/or supervise the development of in vitro and in vivo functional assays to assess the delivery, safety and efficacy of our therapeutics
  • Present data to management, cross-functional project teams, and external partners
  • Propose and integrate methodologies, technologies, and hypotheses to advance the projects and deliver high-quality data
  • Hire and mentor additions to the team
  • Design and execute preclinical in vitro experiments to support optimization and drug product development, and employ relevant rodent or non-human primate models to characterize gene editing candidates
Desired Qualifications
  • Familiarity with CRISPR technologies and gene therapy delivery systems (viral and non-viral)
  • Experience with advanced primary in vitro cell culture of iPSCs, myoblast, myotubes and cardiomyocytes
  • Experience with iPSCs differentiation into myoblast, myotubes and cardiomyocytes
  • Experience with transfection methods of relevant cell types (Lipofection, nucleofection, AAV transduction)
  • Immunostaining of in vitro cultured myoblast, myotubes and cardiomyocytes
  • Experience with primer design, cloning strategies, PCR, library preparation for next generation sequencing, RNA handling and cDNA library generation, qPCR and/or ddPCR
  • A strong foundation in experimental documentation, basic statistical analysis and data presentation
  • Bioassay development and optimization
  • Excellent oral and written communication skills
Mammoth Biosciences

11-50 employees

CRISPR disease detection biotech company