Scientist I/II

Position Description

The Scientist I/II will work with Tr1X’s Vector and Molecular Biology team to develop a cell engineering infrastructure to enable proof-of-concept studies for pipeline program advancement. This role offers exposure to company leadership, prime mentoring and scientific training, and an opportunity to be a high-impact contributor to the company’s mission and vision. The successful candidate will have demonstrable expertise in molecular cloning, and the design, execution, and validation of gene editing experiments, and will be expected to contribute both technical and scientific expertise toward program and project advancement.

The Impact You’ll Make

• Build – a robust cell engineering infrastructure to enable proof-of-concept experiments in primary immune cells
• Perform over-expression, knock-out, and knock-in experiments in cell culture and primary cells
• Design and produce expression and viral plasmids in support of program goals.
• Maintain detailed and highly organized documentation of experimental procedures, SOPs, data, and generated products.
• Analyze data in a timely manner and present findings at team/departmental meetings.
• Demonstrate advanced knowledge of principles and techniques in molecular cloning, gene editing, and cell culture
• Keep up to date - on industry knowledge and advancements; provide input on optimization of design, process, or capability.
• Contribute – Be a subject matter expert and contribute to project direction, prioritization and mitigation strategies.
• Mentor junior Research Associates to help them reach the next level
• Collaborate - and work effectively with Discovery Biology, Translational Sciences and CMC teams.

The Team – Yes You Belong!

We are building a diverse, bold, agile, and collaborative organization where each team member works closely and communicates respectfully with each other. Tr1X leadership has high expectations for themselves and for you (and so should you!): we expect the company and all its employees to be deeply focused on bringing Tr1X’s therapies to patients at an accelerated pace.

Key attributes to this role include attention to detail, integrity, ability to multitask and to adjust to changing priorities, and a willingness to dig in and “get things done.”

You have:

• A PhD in molecular biology, biochemistry, virology, microbiology, or related field with a 2+ years of relevant industry experience
• Strong expertise in the design, execution, and validation of CRISPR/Cas9 gene editing experiments (knock-outs and knock-ins)
• Expertise in molecular cloning techniques (PCR, Restriction digesting, Gibson/Infusion assembly, gel electrophoresis, plasmid prepping)
• Skilled with primer design, Sanger sequencing, and molecular biology and sequence analysis software, such as SnapGene.
• Experience with western blotting or similar protein expression assays
• Expertise in multicolor flow cytometry in primary cells and analysis
• Experience with GraphPad Prism and Microsoft Office Suite programs for data analysis, presentations and report and protocol writing.
• High-level capability with mammalian tissue culture and primary T-cell culture
• Ability to interpret results analytically in a timely manner and troubleshoot technical problems.
• Great attention to detail. Agile and flexible mindset yet highly organized.
• Ability to prioritize multiple tasks, work under various timeline pressures, drive tasks to completion, and anticipate and proactively develop mitigation strategies.
• Previous experience with electronic lab notebooks (ELN).
• The ability to perform in a start-up environment.

Optimally you also have

• Working knowledge of lentiviral vector expression platforms and industrial process development.
• Previous cell therapy experience.
• Hands-on experience supervising and mentoring junior personnel

You are

• Legally entitled to work for any employer in the US.

We offer

In addition to a competitive compensation package with stock options, Tr1X offers comprehensive benefits for our employees and their families including:

• Health insurance
• Vision Insurance
• Dental Insurance
• Flexible PTO
• 401k with generous employer match