E11 Bio Spatial Omics Scientist

Salary Range: 82000 to 141000 (Currency: USD) (Pay period: per-year-salary)

Responsibilities

• Develop proteomics methods (e.g. based on oligo-conjugated antibodies) for highly multiplexed detection of antibodies in expansion microscopy using combinatorial imaging.
• Develop robust signal amplification methods (e.g. rolling circle amplification, hybridization chain reaction) for detection of protein-based cellular barcodes and molecular markers at proteomic scale in swellable hydrogels.
• Design probe sets and combinatorial imaging experiments (e.g., in situ sequencing, MERFISH) compatible with expansion microscopy to detect cell barcodes in brain tissue.
• Integrate and apply automatic liquid handling and automatic fluorescence imaging methods in a robust and reproducible pipeline.
• Process and analyze multiplexed image volumes, including stitching and registration across imaging cycles, and feature detection, decoding and classification.
• Work closely with experimental and computational scientists at E11 and through external partnerships to collaboratively design experiments, analyze experimental data, and interpret results.
• Maintain excellent laboratory notebook practices, prepare reports and manuscripts, and present results internally and externally, and source new techniques from the scientific community.

Required Qualifications

• PhD in biology (e.g. biophysics, bioengineering, molecular biology) or a related field in science and/or engineering, or equivalent working experience and track record.
• Scientist I: 0-3 years of experience post-PhD, or equivalent industry track record; Scientist II: 3-6 years of experience post-PhD, or equivalent industry track record.
• Experience with 3D spatial transcriptomics (e.g. in situ sequencing and/or multiplexed hybridization) and/or spatial proteomics (e.g. multiplexed DNA-barcoded antibodies).
• Extensive experience with spatial methods/protocol development, with emphasis on DNA-based readout such as HCR or RCA.
• Experience with fluorescence microscope modalities, including spinning-disc confocal microscopy.
• Experience with molecular biology laboratory techniques, e.g. cell and tissue culture, molecular cloning, quantitative PCR, immunohistochemistry, etc.

Preferred Qualifications

• Experience with expansion microscopy and/or hydrogel embedding for expansion microscopy.
• Experience with fluidics for multiplexing, microfluidics, and automation.
• Experience with computational image analysis methods for combinatorial imaging
• Excellent communication skills, record keeping ability, and attention to detail.
• Excellent interpersonal and collaboration skills.
• Strong record of publications in peer reviewed scientific journals.

You will love this job if…

• You are excited by applying cutting edge spatial ‘omics technology to an effort that could broadly benefit humanity.
• You are driven by mission and enjoy working among a team focused on a shared audacious goal.
• You enjoy the energy of a small team and the exploration of a wide open, uncharted space.

We offer

• An opportunity to help change the world and work with some of the most talented and collaborative experts across scientific fields.
• An opportunity to gain experience on a field-defining moonshot effort.
• Excellent medical, dental, and vision insurance through a PPO plan; parental leave.
• Generous time off + paid holidays.
• Ample healthy and energizing food including daily lunches.
• Wellness allowance for fitness and wellness activities.