Job Description Summary
Under the direct supervision of the Principal Investigator, a Research specialist III is needed to support glycobiology research for the analysis of human and mouse, tissues, cells and serum. Responsible for supporting sample preparation and analysis for MALDI-glycan imaging, PCR/qPCRs, Western Blots, GlycoTyper, cell culture and drug studies.
Entity
Medical University of South Carolina (MUSC - Univ)
Worker Type
Employee
Worker Sub-Type
Research Grant
Cost Center
CC001032 COM PHARM Administration CC
Pay Rate Type
Salary
Pay Grade
University-06
Pay Range
46,655.00 - 66,488.00 - 86,321.000
Scheduled Weekly Hours
40
Work Shift
Job Description
Duties Performed:
30%. Preparation and analysis of human tissues for glycan analysis. This includes cataloging slides that arrive from collaborators, performing antigen retrieval of slides, preparing slides for glycan imaging using the appropriate methods, using an HTX sprayer to apply specific enzymes for analysis and perform MS analysis of tissue. Subsequently, and independently, perform data analysis to determine which glycans have altered, and help in preparation of manuscript.
30%. Preparation of human serum for glycan analysis using the GlycoTyper method. Identify specific antibodies for utilization, which includes determining the correct antibodies that can be used on amine reactive slides. Prepare slides for glycan imaging, use the HTX sprayer to apply specific enzymes for analysis and perform MS analysis of antibody captured proteins. Subsequently, and independently, perform data analysis to determine which glycoproteins have altered glycans and and help in preparation of manuscript.
20%. Asist in maintaining mouse colony and genotyping. This job requires the individual to be able to step in and help others in mouse work. There is also the potential that based upon the results, this position may require the development of independent mouse work as well.
10% Maintains laboratory equipment by performing checklist systems suitability and required maintenance.
10% Maintain a detailed notebook of experiments performed and location of where data is stored.
MUSC Minimum Training and Experience Requirements:
A bachelor's degree in chemistry, biology, or other natural, life or health care science directly related to the assigned area of research andtwo yearsadvanced journel-level work experience in a clinical or research setting.
Minimum qualifications: MD, PhD, or BSc
In case of MD or PhD (in Biochemistry molecular/cell biology, or related field); having at least one first-author publication in the field of glycobiology.
In case of BSc; experience in Mass Spectrometry, glycobiology and utilization of serum, tissue from both mouse and humans for analysis.
Additional Job Description
Minimum Requirements: A bachelor's degree in chemistry, biology, or other natural, life or health care science directly related to the assigned area of research and two years advanced journel-level work experience in a clinical or research setting. Physical Requirements: (Note: The following descriptions are applicable to this section: Continuous - 6-8 hours per shift; Frequent - 2-6 hours per shift; Infrequent - 0-2 hours per shift) Ability to perform job functions in an upright position. (Frequent) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb, using step stool. (Infrequent) Ability to work in temperature extremes (32° F to 100° F) when required due to assignments in cold rooms and/or foreign countries. (Infrequent) Ability to work in confined/cramped spaces. (Continuous) Ability to bend at the waist. (Frequent) Ability to twist at the waist. (Frequent) Ability to fully use both hands/arms. (Continuous) Ability to perform repetitive motions with hands/wrists/elbows and shoulders. (Frequent) Ability to fully use both legs. (Frequent) Ability to reach in all directions, stretch, bend, while moving items/materials from both sitting and standing positions. (Frequent) Ability to maintain good olfactory sensory function/distinction. (Continuous) Ability to lift materials up to 20 lbs., unassisted. (Infrequent) Ability to lift and carry 4 liter waste material bottles a distance of 30 feet, unassisted. (Frequent) Ability to lift and carry 25-30 lbs. jugs of liquid a distance of 100 feet, unassisted. (Infrequent) Ability to lift file boxes, from floor level to counter tops, unassisted. (Frequent) Ability to push/ Ability to lift 40 lbs. centrifuge rotors from floor level to height of 40 inches, then down 20 inches into centrifuge drum, unassisted. (Infrequent) Ability to lift 25 lbs. boxes, from floor level to maximum height of 72 inches, unassisted. (Infrequent) Ability to push/pull file boxes from one location to another at floor level. (Frequent) Ability to maneuver heavy equipment on bench tops, 50 lbs. or greater, unassisted. (Frequent) Ability to perform gross motor functions with frequent fine motor movements. (Continuous) Ability to maintain 20/40 vision, corrected, in one eye or with both eyes. (Continuous) Ability to match or discriminate between colors, normally. (Continuous) Ability to hear and recognize various alarms emitting from work area systems. (Continuous) Ability to hear and/or understand conversations. (Continuous) Ability to work in a darkroom for processing film, etc. (Continuous) Ability to be qualified physically for respirator use, initially and annually. (Continuous) Additional New Requirements: Ability to work rotating shifts as required. Ability to work overtime as required. Ability to learn and use new processes, tools and equipment as required.
If you like working with energetic enthusiastic individuals, you will enjoy your career with us!
The Medical University of South Carolina is an Equal Opportunity Employer. MUSC does not discriminate on the basis of race, color, religion or belief, age, sex, national origin, gender identity, sexual orientation, disability, protected veteran status, family or parental status, or any other status protected by state laws and/or federal regulations. All qualified applicants are encouraged to apply and will receive consideration for employment based upon applicable qualifications, merit and business need.
Medical University of South Carolina participates in the federal E-Verify program to confirm the identity and employment authorization of all newly hired employees. For further information about the E-Verify program, please click here: http://www.uscis.gov/e-verify/employees
Lab & Research (1)