Position Overview:

We are currently seeking a motivated self-starter and experienced scientific technical leader with expertise developing, validating, and implementing molecular and cell biology assays for research. Experience with developing biomarker assays would be highly desirable. The candidate will join and contribute to a multidisciplinary program team focused on developing novel engineered prime edited ex vivo cell and in vivo therapies. The candidate will apply her/his deep expertise to develop and implement molecular, cell-based, and immunologic assays including genotyping assays, immunogenicity assays, and functional assays that quantify restoration of protein activity after prime editor-mediated gene correction. 

Qualifications:

• PhD plus 0-2 years’ research experience or BS/MS plus 6-8 years’ relevant research experience.
• Experience with primary human cell culture and cell-based in vitro study design and method optimization.
• Experience in assay development, validation, and implementation to support target candidate validation for pre-GLP research.
• Proficiency in molecular biology skills, including primer design, cloning, PCR, qPCR, RT-qPCR, ddPCR, DNA, and RNA isolation.
• Proficiency in cell culture, including primary cells and flow cytometry.
• Experience developing biomarker/clinical biomarker assays would be highly desirable.
• Ability to work effectively on cross-functional teams with a willingness to roll-up-sleeves to get things done.
• Strong oral, written, and verbal communication skills and flexibility to adapt to a dynamic environment.
• Demonstrated maturity to influence, work independently, and cohesively as a self-aware team player.

Primary responsibilities:

• Designing, validating, and running assays to demonstrate Prime Editing dependent phenotypic changes.
• Designing, validating, and running immunological assays to both QC Prime Editing components in vitro and assess immune response in vivo.
• Build assay to quantify amounts of Prime Editor components in different tissues post in vivo delivery.
• Generating, analyzing, and interpreting flow cytometry and other complex data sets.
• Coordinating and working with various other groups in a matrixed environment, as well as external CROs.
• Maintaining detailed documentation of experiments in ELN system.
• Presenting data and findings to internal and external scientific groups.