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Scientist/Senior Scientist
Lentiviral Vectors & Molecular Biology
Confirmed live in the last 24 hours
Locations
Belmont, MA, USA
Experience Level
Entry
Junior
Mid
Senior
Expert
Desired Skills
Data Analysis
Requirements
  • Education in biochemistry/molecular biology or related discipline
  • Biotech / pharma experience of > 1-3 years with a PhD or 4-5 years with a MS
  • Proven track record in hands-on lentiviral vector production and application of modern Molecular Biology technologies
  • Experience with genome engineering (e.g. CRISPR/Cas9, ZFN) and DNA assembly methods (e.g. Gibson, Golden Gate)
  • Hands-on expertise with a variety of current molecular biology and cell culture procedures (DNA cloning, synthetic biology, NGS, RNA, gene editing, Flow Cytometry)
  • Experience with viral application in vivo and/or lab automation are a plus
Responsibilities
  • Design, cloning and production of lentiviral vectors and libraries for screening of immune receptors in T Cells
  • These vectors may express T Cell Receptor (TCR) genes, protein co-factors or reagents for genome engineering (e.g. CRISPR/Cas9)
  • Ownership of the process from digital data analysis to sequence assembly, library generation, QC and sequencing with the help of our NGS core team
  • Mentoring and coordination of research associates as direct reports
  • Collaboration with research teams from Bioinformatics, Immunology, Discovery, Translational Sciences and Technical Operations
  • Contribution to immune receptor screening and characterization
  • Select and apply the best protocols to consistently generate high quality vectors for the purpose
  • Design and execute experiments to determine cellular phenotypes after viral transduction of human primary cells (e.g. expression levels, mRNA splice variants, phenotypic changes)
  • Cooperation with CROs to outsource protocols where practical; troubleshoot technical issues to ensure high quality results
  • Analyze and report experimental results verbally and in writing
Abata Therapeutics

11-50 employees

T-cell transformation medicine company