Research Specialist 3 - Department of Pharmacology & Immunology

Job Description Summary

We seek a fulltime Research Specialist III for the experimental bone marrow transplantation laboratories and tumor immunology laboratory of Dr. Paczesny in the Hollings-NCI designated Cancer Center. The primary responsibilities will include pre-clinical models of Graft-versus-Host disease and acute myeloid leukemia. Specifically, this will involve daily observation and scoring of animals, procurement and breeding of experimental animals and coordination of their housing and care. General laboratory responsibilities will include supply procurement, document maintenance and work closely with IACUC and various other agencies to ensure safety and regulatory compliance for the laboratories. In addition, the candidate will actively participate in setting up transplants (including marrow isolation, spleen cell purification, irradiation and /or chemotherapy and small animal handling/injection) as well as in vitro cellular immunology experiments including CTL assays, MLR cultures, ELISAs, and FACS analysis including spectral flow.

Entity

Medical University of South Carolina (MUSC - Univ)

Worker Type

Employee

Worker Sub-Type​

Research Grant

Cost Center

CC001032 COM PHARM Administration CC

Pay Rate Type

Salary

Pay Grade

University-GEN09

Pay Range

53,142.00 - 71,706.00 - 90,372.000

Scheduled Weekly Hours

40

Work Shift

Job Description

Job Duties:

Perform immunologic experiments on murine models (e.g., harvest organ, digest, flow cytometry, confocal, etc) (20%).

Immunology experimentation employing various techniques, including isolation and purification of T cells, and regulatory T cells. Sort T cells and other hematopoietic or immunes cells.

Perform isolation of cells, cell culture and in vitro functional assays (20%).

Harvest murine organs, including spleen, liver, bone marrow, and lungs. Perform tissue digestion and preparation of single-cell suspensions. Perform isolation and purification of T cells, and regulatory T cells. Sort T cells and other hematopoietic or immunes cells. Maintain cell lines; viability, cytotoxicity assay; transfection and infection, etc.

Perform routine handling of mice including monitoring, treatments, and experimental procedures) (20%).

Assist with breeding, colony management, and genotyping of transgenic. Maintain accurate animal records and ensure compliance with institutional animal care and biosafety protocols. Assist with intravenous and intraperitoneal injections of novel therapeutics (cellular of antibody-based).

Records and analyzes results of research experiments (15%).

Collect research experimental data and analyze results and generate figures.

Assist with daily lab operations including routine maintenance of and experimentation in mice (15%).

The selected candidate will assist with daily lab operations including stocking consumable items and reagents, making buffers, and aliquoting reagents. Fill liquid nitrogen to keep frozen cells alive.

Autoclave the biologic trash and prepare and maintain lab work (e.g., fill and autoclave tips, clean hood, and incubator).

Facilitate general maintenance of the research space by working with MUSC to ensure that the laboratory area is maintained and that repairs, waste disposal, and cleaning needs are addressed in a timely manner.

Additional duties assigned by the Principal Investigator (10%).

This will include helping refine or prepare lab protocols, maintain IBC and IACUC protocols up to date. Train new lab members for basic experiments.

Preferred Requirements:

Candidates with a master degree and minimal 7 years’ experience in the field of immunology, microbiology, molecular biology, cell biology or biochemistry are encouraged to apply. Ability to work independently and good management skills are required. This position requires proficiency with Microsoft Word, Powerpoint, Excel, GraphPad and Endnote.

Additional Job Description

Minimum Requirements: A bachelor's degree in chemistry, biology, or other natural, life or health care science directly related to the assigned area of research and two years advanced journel-level work experience in a clinical or research setting. Physical Requirements: (Note: The following descriptions are applicable to this section: Continuous - 6-8 hours per shift; Frequent - 2-6 hours per shift; Infrequent - 0-2 hours per shift) Ability to perform job functions in an upright position. (Frequent) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb, using step stool. (Infrequent) Ability to work in temperature extremes (32° F to 100° F) when required due to assignments in cold rooms and/or foreign countries. (Infrequent) Ability to work in confined/cramped spaces. (Continuous) Ability to bend at the waist. (Frequent) Ability to twist at the waist. (Frequent) Ability to fully use both hands/arms. (Continuous) Ability to perform repetitive motions with hands/wrists/elbows and shoulders. (Frequent) Ability to fully use both legs. (Frequent) Ability to reach in all directions, stretch, bend, while moving items/materials from both sitting and standing positions. (Frequent) Ability to maintain good olfactory sensory function/distinction. (Continuous) Ability to lift materials up to 20 lbs., unassisted. (Infrequent) Ability to lift and carry 4 liter waste material bottles a distance of 30 feet, unassisted. (Frequent) Ability to lift and carry 25-30 lbs. jugs of liquid a distance of 100 feet, unassisted. (Infrequent) Ability to lift file boxes, from floor level to counter tops, unassisted. (Frequent) Ability to push/ Ability to lift 40 lbs. centrifuge rotors from floor level to height of 40 inches, then down 20 inches into centrifuge drum, unassisted. (Infrequent) Ability to lift 25 lbs. boxes, from floor level to maximum height of 72 inches, unassisted. (Infrequent) Ability to push/pull file boxes from one location to another at floor level. (Frequent) Ability to maneuver heavy equipment on bench tops, 50 lbs. or greater, unassisted. (Frequent) Ability to perform gross motor functions with frequent fine motor movements. (Continuous) Ability to maintain 20/40 vision, corrected, in one eye or with both eyes. (Continuous) Ability to match or discriminate between colors, normally. (Continuous) Ability to hear and recognize various alarms emitting from work area systems. (Continuous) Ability to hear and/or understand conversations. (Continuous) Ability to work in a darkroom for processing film, etc. (Continuous) Ability to be qualified physically for respirator use, initially and annually. (Continuous) Additional New Requirements: Ability to work rotating shifts as required. Ability to work overtime as required. Ability to learn and use new processes, tools and equipment as required.

If you like working with energetic enthusiastic individuals, you will enjoy your career with us!

The Medical University of South Carolina is an Equal Opportunity Employer. MUSC does not discriminate on the basis of race, color, religion or belief, age, sex, national origin, gender identity, sexual orientation, disability, protected veteran status, family or parental status, or any other status protected by state laws and/or federal regulations. All qualified applicants are encouraged to apply and will receive consideration for employment based upon applicable qualifications, merit and business need.

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