U

University of Kansas Medical Center

Health sciences campus in Kansas City, KS

Research Assistant - BMB Research

Full-Time
$22.88 - $33.17/hr
Mid
Bachelor's
Kansas City, MO, USA
In Person
Academic & Institutional ResearchLife Sciences Research

About the job

Requirements
  • Four years of experience in protein biochemistry, including expression and purification of recombinant proteins and functional analysis of proteins; education may substitute for experience on a year-for-year basis.
Responsibilities
  • Generate, purify, and organize expression plasmids for proteins of interest using miniprep and maxiprep protocols.
  • Perform cell culture techniques, including routine cell maintenance, transient transfections, preparation of nuclear extracts, and utilization of CRISPR/Cas9.
  • Assist with fluorescent imaging techniques in biochemical and live-cell contexts.
  • Collaborate with fellow lab members, including graduate students and technicians, to achieve research objectives.
  • Attend and maintain compliance with University and Department of Biochemistry and Molecular Biology orientation and training sessions, follow assigned duties, and attend regularly scheduled research team meetings.
  • Conduct physical, chemical, biological, and other research laboratory tests, experiments, and determinations to obtain research data; compile, analyze, document, and draw tentative conclusions from experimental results.
  • Confer with the principal investigator to review work assignments and develop plans for research experiments.
  • Make minor modifications to existing laboratory procedures and techniques to meet equipment needs.
  • Review laboratory methods manuals, scientific journals, abstracts, and other literature for information applicable to research experiments.
Desired Qualifications
  • A bachelor's or equivalent degree in Biochemistry, Molecular Biology, Cell Biology, or a related field.
  • Experience in cell biology, including CRISPR/Cas9 approaches, transient transfections, and PCR characterization.
  • Experience in microscopy or fluorescent proteins.
  • Experience in cell culture and sterile techniques.
  • General experience in molecular biology techniques, including SDS-PAGE, PCR, and agarose gels.

About the company

U

University of Kansas Medical Center

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University of Kansas Medical Center is the University of Kansas academic health sciences campus in Kansas City, Kansas. Its schools provide degree-granting education in health professions, medicine, and nursing.

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