Scientist 2/Senior Scientist - Expansion Microscopy Labeling and Multiplexing

The Mindspan Institute is a new non-profit research institute in Cambridge, MA, aimed at building novel tools to analyze the human brain, and scaling them to study its molecules and wiring. We interpret how they contribute to brain functions and dysfunctions, and share our tools and discoveries as freely as possible, to advance human health.

The role

This is a hands-on job optimizing, and applying, staining technologies for the multiplexed investigation of expansion microscopy (ExM)-processed brain tissue.  The role involves screening binders to specific molecular targets, implementing fluorophore conjugation strategies to achieve target multiplexing, and developing tissue-wide ultrastructure staining methods.  Efforts will be put towards optimizing, applying, and scaling existing methods.  You will report to the Head of Molecular Labeling Technologies, and work, as needed, with other Mindspan teams (Scalable Chemistry, Optical Physiology, Microscopy and Optics, Automation and Industrialization, and Computation and Analysis), using logic and evidence to advance your work so as to boost performance for all.

The position is intended for someone who wants to uncover the 3D molecular details of the brain with cutting-edge detail.  Its multidisciplinary nature will require someone who enjoys learning about and solving problems in chemistry, microscopy, and biology.   It also requires teamwork, systematic documentation, analysis, and iterative problem solving, to arrive at working protocols, apply them, and share them.

What you'll own

Binder discovery. You will screen and discover binders (e.g., antibodies) to image molecular targets in expanded brain tissue.  

Bioconjugation. For the purposes of increasing molecular multiplexing and achieving high signal intensities, you will modify binders with fluorescent molecules as well as other chemistries in a fashion that leads to multiplexed readouts.

Staining chemistry. In addition to staining for specific molecular targets, you will develop and apply technologies that can stain and investigate cellular morphologies at scale, to delineate brain wiring.

Imaging. Characterization of staining will be performed through fluorescence microscopy.   You will perform image analysis to quantify results to ensure desired outcomes are obtained.

Documentation and training. You will keep protocol records that others can execute along with the reasoning behind each parameter. You will collaborate with the Automation and Industrialization team to translate validated protocols into robotic execution and contribute protocols and data to the open resources we train others on.

Culture we're building

The disorders of the brain affect over 1,000,000,000 people around the world: Alzheimer’s, Parkinson’s, stroke, and other conditions. We operate with a sense of urgency, even as we look to understand the brain at its most fundamental levels.

We are hypercollaborative, and focused on impact over short-term markers of productivity. Creativity and failure are the steppingstones to scale, and working together with “strong opinions, weakly held” is our style.

We solve real engineering problems the best way we can: inventing fast when we have to, and using good existing solutions where they exist. We believe hard problems only yield to many disciplines working together, so collaboration is not optional.

We expect people to explore the unknown, which means making mistakes daily, owning them, and learning from them to improve and excel.

We are recruiting a team of scientists and engineers who exemplify all these values.

What we're looking for

You have a Ph.D. in chemistry, chemical engineering, materials science, bioengineering, neuroscience, or a related field.  You have a strong foundation in conventional immunostaining of tissues as well as experience in advanced, multiplexed methods.  You have experience with bioconjugation and fundamental skills in synthetic and polymer chemistry.

You should be experienced with fluorescence microscopy techniques and fluorophore chemistry.  Experience with expansion microscopy and tissue clearing techniques is a plus.  Also, you should be comfortable with routine image analysis (Python, Fiji, etc.) and be able to quantitatively evaluate microscopy data.  You have a track record of independent method development where the benchmarking was as thoughtful as the invention. You are quick and agile, yet able to focus; creative, yet disciplined.

You are detail oriented and have exceptional organizational, planning, interpersonal, and communication skills. You are good at writing, and can work independently as well as lead and manage.  You can prioritize and multitask as needed. Self-motivation, commitment to high quality standards, and a service-oriented mindset are all key. You have good judgment, balancing flexibility and focus, action and planning, speed and thoughtfulness, as appropriate. You are open to giving and receiving feedback, constructively and thoughtfully, to advance scientific missions and personal growth.

This is an in-person job. Flexible hours may be required at times.

What we offer

The chance to build the staining pipeline of a new research institute alongside some of the most adventurous scientists in the field, with real autonomy and a direct line to the people setting the scientific agenda. You will get to contribute to addressing one of the most important unmet medical needs of our time, as part of the initial team. You will learn lots of things that probably cannot be learned any other way, as you solve problems, build, and work with others. As Mindspan grows, we expect this role to grow with it. We are excited to invest in someone who wants to take on increasing responsibility over time.

Comprehensive health, dental, vision, and family benefits, and a matching retirement contribution.

Competitive salary $120,000 to $159,000, depending on experience.