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Medical University of South Carolina

Health sciences university in Charleston, SC

Research Specialist 2 - Department of Medicine, Division of Infectious Diseases

Life Sciences ResearchConfirmed live in the last 24 hours
Full-timeCharleston, SC, USANo salary listedIn-person

About the Job

Requirements

  • A bachelor's degree in chemistry, biology, or another natural, life, or health care science and one year of work experience directly related to the assigned research area are required.
  • Prior laboratory experience is required.
  • Experience with primary and established cell line culture techniques, including cell expansion, transfection, and characterization.
  • Proficiency with molecular biology techniques, including DNA and RNA isolation, PCR/qRT-PCR, and molecular cloning.
  • Experience with protein and cellular assays, including western blotting, flow cytometry, and fluorescent microscopy.
  • Familiarity with analysis and interpretation of biologic datasets, including proteomic and transcriptomic data.
  • Ability to perform job functions in an upright, seated, and walking/mobile position; climb using a step stool; work in temperature extremes when assigned, confined or cramped spaces, bend and twist at the waist, fully use both hands/arms and both legs, perform repetitive motions, and reach, stretch, bend, and move items/materials from sitting and standing positions.
  • Ability to maintain olfactory sensory function, lift and carry specified materials and waste bottles, lift and carry jugs, file boxes, centrifuge rotors, and other equipment as specified, push and pull file boxes, and maneuver heavy equipment on bench tops.
  • Ability to perform gross motor functions with frequent fine motor movements; maintain corrected 20/40 vision in at least one eye; discriminate colors; hear and recognize alarms and understand conversations; work in a darkroom; and qualify physically for respirator use initially and annually.
  • Ability to work rotating shifts and overtime as required, and to learn and use new processes, tools, and equipment as required.

Responsibilities

  • Maintain, expand, and characterize mammalian primary cells and cell lines under sterile culture conditions.
  • Perform cell-based assays, treatments, genetic manipulations, and other experimental procedures.
  • Perform cellular and molecular biological techniques, including RNA/DNA isolation, DNA cloning, PCR/qRT-PCR, western blotting, flow cytometry, and fluorescent microscopy.
  • Accurately document experimental procedures and data in an electronic notebook.
  • Analyze data and perform statistical analyses using appropriate software such as GraphPad.
  • Participate in weekly laboratory meetings.
  • Assist with preparations for presentations and publications.
  • Assist with day-to-day laboratory operations, including inventory management, selection and ordering of reagents, and equipment maintenance.
  • Perform complex biological procedures and analyses in a basic science and translational laboratory under the supervision of the Principal Investigator and Staff Scientist.
  • Optimize and troubleshoot experimental protocols.
  • Work occasional weekends.

About the Company

Medical University of South Carolina

Health sciences university in Charleston, SC

Medical University of South Carolina is a public academic health sciences university in Charleston, South Carolina. It offers bachelor's, master's, doctoral, and professional degrees through six colleges in dental medicine, graduate studies, health professions, medicine, nursing, and pharmacy.

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